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cd45 1 pe cy7  (Thermo Fisher)


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    Thermo Fisher cd45 1 pe cy7
    Cd45 1 Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd45 1 pe cy7/product/Thermo Fisher
    Average 97 stars, based on 264 article reviews
    cd45 1 pe cy7 - by Bioz Stars, 2026-02
    97/100 stars

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    Expression of p38α D176A+F327S did not result in noticeable adverse outcomes or pathological changes in the lungs of p38αSFTPC mice. A , p38αSFTPC-hetero ( top panel ) and p38αSFTPC-homo mice ( bottom panel ) were provided with either a Dox-supplemented or a regular diet and weighed every 2 weeks for up to 12 months. The average weight of each group over the 12 months is presented (n = 12–36). B , lung sections obtained from p38αSFTPC-homo mice provided with a Dox-supplemented or a regular diet for 1, 3, 6, and 12 months were H&E stained. Sections were imaged at 100x magnification and representative images are shown (n = 3). C , counts of cells obtained from BAL fluid of p38αSFTPC-homo mice provided with a Dox-supplemented or regular diet for 1, 3, and 6 months (n = 8). D , FACS gating strategy to identify immune cell subsets in the BAL fluid. Immune cells are defined as <t>CD45</t> + . After gating on immune cells, AMϕ are defined as CD45 + CD11c + Siglec F + , while eosinophils are defined as CD45 + CD11c - Siglec F + . Neutrophils are defined as CD45 + CD11b + Gr-1 + . After gating on AMϕ, M1-like AMϕ are defined as iNOS + , while M2-like AMϕ are defined as Arg-1 + . E , BAL fluid cell composition from p38αSFTPC-hetero mice ( left panel ; n = 9) and differential counts of BAL fluid cells from p38αSFTPC-homo mice ( right panel ; n = 7–8) fed with a Dox-supplemented or regular diet for up to 6 months. The total percentage of CD45+ immune cells is represented in the leftmost column , which is further broken down into AM, eosinophil (Eos), and neutrophil (Neu) subpopulations. F , level of cytokines and chemokines in BAL fluid from p38αSFTPC-homo mice provided with a Dox-supplemented or regular diet for 2 weeks, and from WT mice provided with a Dox-supplemented diet (n = 3–4). AMϕ, alveolar macrophages; Arg1 + , arginase-1 + ; BAL, bronchoalveolar lavage; Dox, doxycycline; FACS, fluorescence-activated cell sorting; iNOS, inducible nitric oxide synthase.
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    Expression of p38α D176A+F327S did not result in noticeable adverse outcomes or pathological changes in the lungs of p38αSFTPC mice. A , p38αSFTPC-hetero ( top panel ) and p38αSFTPC-homo mice ( bottom panel ) were provided with either a Dox-supplemented or a regular diet and weighed every 2 weeks for up to 12 months. The average weight of each group over the 12 months is presented (n = 12–36). B , lung sections obtained from p38αSFTPC-homo mice provided with a Dox-supplemented or a regular diet for 1, 3, 6, and 12 months were H&E stained. Sections were imaged at 100x magnification and representative images are shown (n = 3). C , counts of cells obtained from BAL fluid of p38αSFTPC-homo mice provided with a Dox-supplemented or regular diet for 1, 3, and 6 months (n = 8). D , FACS gating strategy to identify immune cell subsets in the BAL fluid. Immune cells are defined as CD45 + . After gating on immune cells, AMϕ are defined as CD45 + CD11c + Siglec F + , while eosinophils are defined as CD45 + CD11c - Siglec F + . Neutrophils are defined as CD45 + CD11b + Gr-1 + . After gating on AMϕ, M1-like AMϕ are defined as iNOS + , while M2-like AMϕ are defined as Arg-1 + . E , BAL fluid cell composition from p38αSFTPC-hetero mice ( left panel ; n = 9) and differential counts of BAL fluid cells from p38αSFTPC-homo mice ( right panel ; n = 7–8) fed with a Dox-supplemented or regular diet for up to 6 months. The total percentage of CD45+ immune cells is represented in the leftmost column , which is further broken down into AM, eosinophil (Eos), and neutrophil (Neu) subpopulations. F , level of cytokines and chemokines in BAL fluid from p38αSFTPC-homo mice provided with a Dox-supplemented or regular diet for 2 weeks, and from WT mice provided with a Dox-supplemented diet (n = 3–4). AMϕ, alveolar macrophages; Arg1 + , arginase-1 + ; BAL, bronchoalveolar lavage; Dox, doxycycline; FACS, fluorescence-activated cell sorting; iNOS, inducible nitric oxide synthase.

    Journal: The Journal of Biological Chemistry

    Article Title: A combination of alveolar type 2–specific p38α activation with a high-fat diet increases inflammatory markers in mouse lungs

    doi: 10.1016/j.jbc.2025.108425

    Figure Lengend Snippet: Expression of p38α D176A+F327S did not result in noticeable adverse outcomes or pathological changes in the lungs of p38αSFTPC mice. A , p38αSFTPC-hetero ( top panel ) and p38αSFTPC-homo mice ( bottom panel ) were provided with either a Dox-supplemented or a regular diet and weighed every 2 weeks for up to 12 months. The average weight of each group over the 12 months is presented (n = 12–36). B , lung sections obtained from p38αSFTPC-homo mice provided with a Dox-supplemented or a regular diet for 1, 3, 6, and 12 months were H&E stained. Sections were imaged at 100x magnification and representative images are shown (n = 3). C , counts of cells obtained from BAL fluid of p38αSFTPC-homo mice provided with a Dox-supplemented or regular diet for 1, 3, and 6 months (n = 8). D , FACS gating strategy to identify immune cell subsets in the BAL fluid. Immune cells are defined as CD45 + . After gating on immune cells, AMϕ are defined as CD45 + CD11c + Siglec F + , while eosinophils are defined as CD45 + CD11c - Siglec F + . Neutrophils are defined as CD45 + CD11b + Gr-1 + . After gating on AMϕ, M1-like AMϕ are defined as iNOS + , while M2-like AMϕ are defined as Arg-1 + . E , BAL fluid cell composition from p38αSFTPC-hetero mice ( left panel ; n = 9) and differential counts of BAL fluid cells from p38αSFTPC-homo mice ( right panel ; n = 7–8) fed with a Dox-supplemented or regular diet for up to 6 months. The total percentage of CD45+ immune cells is represented in the leftmost column , which is further broken down into AM, eosinophil (Eos), and neutrophil (Neu) subpopulations. F , level of cytokines and chemokines in BAL fluid from p38αSFTPC-homo mice provided with a Dox-supplemented or regular diet for 2 weeks, and from WT mice provided with a Dox-supplemented diet (n = 3–4). AMϕ, alveolar macrophages; Arg1 + , arginase-1 + ; BAL, bronchoalveolar lavage; Dox, doxycycline; FACS, fluorescence-activated cell sorting; iNOS, inducible nitric oxide synthase.

    Article Snippet: Fluorochrome-labeled antibodies used for flow cytometry: Pe/Cy7 anti-mouse CD45, PE anti-mouse CD170 (Siglec F), APC anti-mouse CD11b, PerCp-Cy5.5 anti-mouse Gr1, APC/Cy7 anti-mouse NOS2 inducible nitric oxide synthase, APC anti-mouse CD206, PerCp-eFluor 710 anti-mouse CD80 (Thermo Fisher Scientific), FITC anti-mouse Arg-1 (R&D Systems), and PB anti-mouse CD11c (BioLegend).

    Techniques: Expressing, Staining, Fluorescence, FACS